📚 Wiki Longevity & Anti-Aging GHK

GHK

✦ Cosmetic / Preclinical
Glycyl-L-Histidyl-L-Lysine (Copper-Free)
Also known as: GHK tripeptide, Glycyl-His-Lys, GHK free peptide
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Quick Summary

GHK (Glycyl-L-Histidyl-L-Lysine) is the copper-free form of the GHK-Cu tripeptide. While GHK-Cu (GHK chelated to copper) is more widely studied for wound healing and tissue repair, GHK alone retains some bioactivity, particularly for signaling collagen synthesis and gene expression changes in fibroblasts.

Skin & Cosmetic Research Use
GHK (Glycyl-L-Histidyl-L-Lysine) is the copper-free form of the GHK-Cu tripeptide. While GHK-Cu (GHK chelated to copper) is more widely studied for wound healing and tissue repair, GHK alone retains some bioactivity, particularly for signaling collagen synthesis and gene expression changes in fibroblasts. It is used in research to isolate the peptide-only effects from copper-chelation effects of GHK-Cu.
Storage Stability
Lyophilized
6–12 months (2–8°C)
Reconstituted
~30 days (2–8°C)
Room temp
Avoid

Mechanism of Action

Gene Expression Modulation

GHK has been shown to alter the expression of over 4,000 human genes, with the majority promoting wound healing, anti-inflammatory, and tissue remodeling pathways. This broad gene-regulatory effect is independent of copper chelation.

Collagen Synthesis Signal

GHK triggers collagen I and III synthesis in dermal fibroblasts through TGF-beta-1 activation and upregulation of ECM remodeling enzymes (MMPs + TIMPs in balanced ratio).

Copper Chelation (GHK-Cu)

The His residue in GHK has high affinity for Cu²⁺. When copper is present, GHK forms GHK-Cu, which has additional wound-healing, angiogenic, and anti-inflammatory properties beyond GHK alone.

Research Summary

Gene Array Studies

Pickart et al. demonstrated GHK's broad transcriptional impact, anti-cancer gene signatures, collagen pathway upregulation, and anti-inflammatory marker suppression across multiple cell lines.

Wound Healing

Both GHK and GHK-Cu accelerate wound closure in animal models. GHK-Cu shows stronger effects; GHK alone provides a baseline peptide-mediated effect.

Skin Aging

Topical GHK reduces fine lines and improves skin texture in small human studies, attributed to collagen synthesis stimulation and improved ECM organization.

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Research Protocols

Topical Use

Formulated at 1–5% concentration in serum or cream base. Apply to target area twice daily. Minimum 8-week assessment period for visible skin outcomes.

Injectable Research

Less common than GHK-Cu for injectable use. When used, typically 1–2 mg subcutaneously into target tissue area.

Storage & Handling

Store at 2–8°C. Protect from light. GHK is more stable than GHK-Cu as it lacks the reactive copper ion. Shelf life 1–2 years when properly stored.


References

  • [1]Pickart L, Margolina A. "Regenerative and protective actions of the GHK-Cu peptide." Int J Mol Sci, 2018.
  • [2]Pickart L, Vasquez-Soltero JM, Margolina A. "GHK peptide as a natural modulator of multiple cellular pathways." Biomed Res Int, 2015.
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Data Sources & External References
Source: peer-reviewed literature  ·  Domain: ascendpeptide.org
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