GHK Dosing & Reconstitution Calculator

Add 2.5 mL of bacteriostatic water to a 5 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 2 mg/mL.

A typical research dose is 2 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.

A 5 mg vial reconstituted with 2.5 mL BAC water yields approximately 2 doses at 2 mg each.

With a 5 mg vial reconstituted in 2.5 mL bacteriostatic water, the concentration is 2 mg/mL. For a 2 mg dose: Volume = 2 mg ÷ 2 mg/mL = 1 mL. Draw to the 100 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.

A GHK dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (5 mg), reconstitution volume (2.5 mL), and target dose to get the precise line to draw on an insulin syringe.

Draw volume (mL) = Dose (mg) divided by Concentration (mg/mL). For a 5 mg vial with 2.5 mL BAC water: concentration = 2 mg/mL. For a 2 mg dose, the ASCEND calculator gives the exact IU line on your syringe instantly.