Storage Stability
Mechanism of Action
HDM2 Surface Targeting
Many cancer cell types express HDM2 on their external plasma membrane surface, an aberrant localization not seen in normal cells. PNC-27's p53 domain binds this surface HDM2, concentrating the peptide on cancer cells.Membrane Pore Formation
The transmembrane penetratin sequence in PNC-27 then inserts into the lipid bilayer, oligomerizes, and forms pores. These pores disrupt membrane integrity, causing rapid necrotic/apoptotic cell death.Selectivity
The selectivity for cancer cells depends on surface HDM2 expression. Cell lines with high surface HDM2 (pancreatic, breast, melanoma) show high sensitivity; normal cell lines are largely spared.Research Summary
In Vitro Data
PNC-27 kills numerous cancer cell lines including pancreatic (MiaPaCa-2), breast, leukemia, and melanoma lines within hours of treatment at low micromolar concentrations. Normal cell lines show minimal toxicity.Pancreatic Cancer Focus
Pancreatic cancer is notoriously resistant to chemotherapy. PNC-27 has shown particular promise in this indication where surface HDM2 expression is consistently high.Animal Models
Limited but encouraging xenograft data shows tumor growth inhibition with PNC-27 treatment in mouse models without significant systemic toxicity.Calculate your PNC-27 dose Vial strength, BAC water, exact syringe draw in IU. Free, no signup. Open Calc →
Research Protocols
Note: PNC-27 is in early preclinical research. No human dosing data exists.
Cell Culture
In vitro research typically uses 10–100 µM concentrations. Cell viability measured at 24–72 hours.Animal Models
Intraperitoneal or intratumoral injection in xenograft models. Dose varies by model (see published protocols).Storage & Handling
Store at -20°C. Reconstitute with DMSO (for cell culture stocks) or sterile saline (animal models). DMSO stock: -80°C long-term. Aqueous solutions: use within 24-48 hours at 4°C.
References
- [1]Bowne WB, et al. "Anticancer activity of the p53 peptidomimetic PNC-27 against pancreatic cancer." Cancer Res, 2008.
- [2]Ambrosone A, et al. "PNC-27, a selective apoptosis-inducing peptide in cancer cells." Anticancer Res, 2018.
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