Storage Stability
Mechanism of Action
Satellite Cell Activation
MGF's unique E-domain peptide directly activates muscle satellite cells (Pax7+ stem cells), causing them to proliferate and differentiate into new myofibers. This is distinct from IGF-1's systemic anabolic effects.Nuclear Translocation
MGF promotes nuclear translocation of MGF receptor complexes, activating gene programs for muscle fiber hypertrophy and regeneration.Complementary to IGF-1 LR3
MGF and IGF-1 LR3 act sequentially in the natural repair process: MGF first activates satellite cells (proliferation phase), then IGF-1 drives their differentiation and protein synthesis (anabolism phase). Combining both in research protocols aims to replicate this sequence.Research Summary
Satellite Cell Studies
In vitro, MGF E-domain peptide increases satellite cell proliferation 2–3x versus controls. In muscle injury models, local MGF expression precedes satellite cell activation within hours.Animal Muscle Repair
Intramuscular MGF injection post-injury significantly accelerates muscle fiber regeneration and increases cross-sectional area at 14 and 28 days post-injury compared to controls.Age-Related Decline
MGF production in response to exercise decreases with age, which may partly explain reduced muscle repair capacity in older individuals.Calculate your MGF dose Vial strength, BAC water, exact syringe draw in IU. Free, no signup. Open Calc →
Research Protocols
Timing (Critical)
Inject within 30–60 minutes post-workout while satellite cells are in the activated state from mechanical damage. The short half-life means injection timing is more important than with longer-acting IGF variants.Route
Intramuscular into the trained muscle for localized effect. Some protocols use SubQ.Dose
100–200 µg post-workout. Can be combined sequentially with IGF-1 LR3 (separate injection 4–8 hours later for differentiation phase).Storage & Handling
Store lyophilized" class="wiki-gloss-link">lyophilized at -20°C. Reconstitute with bacteriostatic water. Use immediately or store at 2–8°C for no more than 2 weeks. Non-PEGylated MGF degrades rapidly at physiological temperature, minimize time at room temperature.
References
- [1]Yang SY, Goldspink G. "Different roles of IGF-1 Ec (MGF) and mature IGF-1 in myoblast proliferation and differentiation." FEBS Lett, 2002.
- [2]Goldspink G. "Mechanical signals, IGF-I gene splicing, and muscle adaptation." Physiology (Bethesda), 2005.
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