How do you inject HMG (human menopausal gonadotropin)?

HMG (human menopausal gonadotropin, e.g., Menopur) is typically injected subcutaneously into the abdomen or thigh using a small-gauge needle. Reconstitute the lyophilized powder with the provided sodium chloride diluent immediately before injection. Rotate injection sites. Some protocols use intramuscular injection (gluteus or thigh). Always follow the prescribed protocol from a fertility specialist.

What is the typical HMG injection dose for fertility treatment?

Standard starting doses for HMG in fertility protocols range from 75 to 225 IU per day, administered subcutaneously or intramuscularly. The cycle typically lasts 7-14 days for controlled ovarian stimulation in ART protocols. For male hypogonadotropic hypogonadism, lower doses (75 IU 2-3x/week) are often used in combination with HCG. Doses are adjusted based on follicle development and hormone response.

Should HMG be injected subcutaneously or intramuscularly?

Both routes are clinically used for HMG. Subcutaneous injection (into the abdomen, thigh, or upper arm) is the most common route for self-administration in fertility protocols as it is easier and less painful. Intramuscular injection (gluteus or upper thigh) is also acceptable and used in some protocols. Subcutaneous is generally preferred for patient self-injection. Follow your fertility clinic instructions.

What is HMG used for?

HMG (human menopausal gonadotropin) is FDA-approved for: (1) controlled ovarian stimulation in women undergoing assisted reproductive technology (ART/IVF); (2) induction of ovulation in anovulatory women; (3) stimulation of spermatogenesis in men with hypogonadotropic hypogonadism. It contains both FSH and LH activity, making it effective for complete gonadal stimulation.

HMG: Mechanism, Dosing & Research Data

Storage Stability

Lyophilized

1–2 years (-20°C)

Reconstituted

~30 days (2–8°C)

Room temp

Avoid

Mechanism of Action

FSH Component

FSH binds FSHR on granulosa cells (females) and Sertoli cells (males). In females, it stimulates follicular recruitment, growth, and estrogen production. In males, it supports Sertoli cell function and is essential for spermatogenesis initiation.

LH Component

LH binds LHR on theca cells (females) and Leydig cells (males). In females, it drives androgen production for estrogen synthesis. In males, it stimulates testosterone production, essential for spermatogenesis completion.

Synergistic Gonadal Stimulation

The combined FSH+LH activity of hMG provides complete gonadal stimulation, which is more effective than FSH alone for males with hypogonadotropic hypogonadism.

Research Protocols

Female ART Protocol

75–225 IU SubQ/IM daily for 7–14 days, monitored with ultrasound and estradiol levels. Dose adjusted per follicular response.

Male Hypogonadotropic Hypogonadism

hMG 75 IU 3x/week combined with hCG 1,000–3,000 IU 3x/week for 3–6+ months to restore spermatogenesis. FSH component supports Sertoli cells while hCG provides LH activity.

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Storage & Handling

Refrigerate at 2–8°C. Do not freeze. Use within expiration date. Reconstituted solution should be used immediately or within same day.

References

[1]Balasch J, Fabregues F. "FSH and hMG in ART: a comparative analysis." Hum Reprod, 2003.
[2]Liu PY, et al. "Recombinant FSH versus hMG for spermatogenesis induction." J Clin Endocrinol Metab, 2009.

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Categories: Hormonal Reproductive FDA Approved WADA Prohibited Fertility ART

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Verified Scientific Data Last audited: June 10, 2026

Data Sources & External References

⬡ PubMed / NCBI, Research Literature ⬡ NCBI, Full-Text Articles ⬡ PubChem, Chemical Database

Source: peer-reviewed literature · Domain: ascendpeptide.org

Evidence	A · FDA Approved
Mol. weight	~34–36 kDa (FSH + LH)
Half-life	FSH ~4 hrs; LH ~1 hr
Peak time	,
Route(s)	Subcutaneous / Intramuscular
Typical dose	75–225 IU per day
Frequency	Daily (fertility protocol)
Cycle	7–14 days (ART protocol)
Storage	2–8°C (refrigerate; do not freeze)
WADA	Prohibited FSH and LH prohibited under S2 (Peptide Hormones) of WADA Prohibited List for males
FDA	Approved FDA approved for infertility treatment in males and females; ovarian stimulation in ART protocols
Research	Approved

HMG