SYRINGE DRAW GUIDE

MGF Draw on Insulin Syringe

Quick answer: For the standard 2 mg + 2 mL reconstitution (1000 µg/mL), a 200 µg MGF dose requires 0.2 mL, which is 20 IU on a 100-unit U-100 insulin syringe.

200 µg draw on 30u, 50u, and 100u U-100 syringes

30-unit (0.3 mL) U-100
05101520253020 IU
Draw to 20 IU
50-unit (0.5 mL) U-100
0102030405020 IU
Draw to 20 IU
100-unit (1 mL) U-100
010203040506070809010020 IU
Draw to 20 IU

Dose ladder - does it fit?

How draw volume changes with dose strength, plus which insulin syringe sizes will accommodate it without overflow.

DoseVolumeIU (100u)30u50u100u
100 µg 0.1 mL 10 IU
200 µg Std 0.2 mL 20 IU
300 µg 0.3 mL 30 IU
400 µg 0.4 mL 40 IU -
600 µg 0.6 mL 60 IU - -

How to read the draw

  1. Read the large numbered marks (10, 20, 30 ...) on the syringe barrel to find your IU target.
  2. Pull the plunger until the rubber stopper aligns with that mark, not the bevel.
  3. Flick the syringe to dislodge air bubbles, then push the plunger to expel any remaining air, ending at the target IU mark.
  4. Confirm the volume reads correctly under good light before injection.
Open MGF Calculator → Insulin syringe reference guide

Frequently asked

How many insulin syringe units is 200 µg of MGF?
For the standard ASCEND reconstitution (2 mg vial + 2 mL BAC water = 1000 µg/mL), a 200 µg dose of MGF requires 0.2 mL, which is 20 IU on a 100-unit U-100 insulin syringe.
Which insulin syringe size is best for MGF?
A 30-unit (0.3 mL) U-100 syringe gives the finest graduations for a 20 IU draw and is the preferred choice when low-dose precision matters. A 50-unit or 100-unit syringe also works.
What is the difference between U-100 and U-40 syringes for peptide use?
U-100 syringes are calibrated so that 100 units = 1 mL. Peptide reconstitution math universally assumes U-100. Never use U-40 syringes for research peptides; the scale will be wrong by a factor of 2.5x and could result in a serious dosing error.
Can I split a single MGF dose across multiple injection sites?
Some researchers split larger draw volumes across two injection sites to reduce local tissue reaction. Splitting does not change the total dose delivered or the math; it only changes site distribution. Rotate sites with each subsequent dose.
Why does the draw value matter for MGF?
MGF dosing is sensitive to precise draw volume. A 1-unit error on a 100-unit insulin syringe corresponds to 1000 µg/mL times 0.01 mL of peptide; a small error in absolute volume can be a large percent error at microgram-scale doses. Use the ASCEND calculator for exact values.

Related

For research use only. Not medical advice. Always use U-100 (not U-40) insulin syringes for peptide reconstitution. ASCEND, ascendpeptide.org