NAD+ Dosing & Reconstitution Calculator

Add 3 mL of bacteriostatic water to a 500 mg vial, injecting slowly down the inside wall. Swirl gently; never shake. This yields a concentration of 166.7 mg/mL.

A typical research dose is 50 mg. Consult the ASCEND calculator for your specific vial concentration and draw volume.

A 500 mg vial reconstituted with 3 mL BAC water yields approximately 10 doses at 50 mg each.

With a 500 mg vial reconstituted in 3 mL bacteriostatic water, the concentration is 166.7 mg/mL. For a 50 mg dose: Volume = 50 mg ÷ 166.7 mg/mL = 0.3 mL. Draw to the 30 line on a 100-unit insulin syringe. You can adjust these values in the ASCEND calculator for any vial size.

A NAD+ dosage calculator converts your vial size and BAC water volume into an exact syringe draw in IU. Enter your vial strength (500 mg), reconstitution volume (3 mL), and target dose to get the precise line to draw on an insulin syringe.

Draw volume (mL) = Dose (mg) divided by Concentration (mg/mL). For a 500 mg vial with 3 mL BAC water: concentration = 166.7 mg/mL. For a 50 mg dose, the ASCEND calculator gives the exact IU line on your syringe instantly.