RECONSTITUTION GUIDE
How Much BAC Water for NAD+?
Quick answer: Add 3 mL of bacteriostatic water to a 500 mg NAD+ vial. This yields a concentration of 166.67 mg/mL. A standard 50 mg research dose then requires 0.3 mL (30 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 50 mg draw volume at common BAC water volumes for a 500 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 500 mg/mL | 0.1 mL | 10 IU |
| 2 mL | 250 mg/mL | 0.2 mL | 20 IU |
| 3 mL Standard | 166.67 mg/mL | 0.3 mL | 30 IU |
| 5 mL | 100 mg/mL | 0.5 mL | 50 IU |
Dose volume at the standard 166.67 mg/mL concentration
How draw volume changes if you vary the dose at the standard 500 mg + 3 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 25 mg | 0.15 mL | 15 IU |
| 50 mg Std | 0.3 mL | 30 IU |
| 75 mg | 0.45 mL | 45 IU |
| 100 mg | 0.6 mL | 60 IU |
| 150 mg | 0.9 mL | 90 IU |
Reconstitution steps
- Wipe the top of both the NAD+ vial and the bacteriostatic water vial with alcohol swabs.
- Draw 3 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the NAD+ vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2-8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- NAD+ 500 mg lyophilized vial
- 3 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 500 mg NAD+ vial?
For the standard ASCEND research protocol, add 3 mL of bacteriostatic water to a 500 mg NAD+ vial. This yields 166.67 mg/mL.
What concentration does 3 mL BAC water give for 500 mg NAD+?
A 500 mg NAD+ vial reconstituted with 3 mL of bacteriostatic water yields 166.67 mg/mL. A 50 mg dose then requires 0.3 mL (30 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for NAD+?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is NAD+ stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2-8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for NAD+?
For a 50 mg dose (30 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.
Can I transport reconstituted NAD+?
Keep it cold in transit. An insulated bag or small cooler with an ice pack holds the 2-8°C range for several hours, which covers most day trips or drives. For longer travel, pack it the same way you would any refrigerated medication, and get it back into a fridge as soon as you arrive. Extended time at room temperature shortens the ~28-day stability window.
Related
NAD+ calculator + tracker, free on iOS Download on the App Store Get appFor research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org