RECONSTITUTION GUIDE
How Much BAC Water for Melanotan II?
Quick answer: Add 2 mL of bacteriostatic water to a 10 mg Melanotan II vial. This yields a concentration of 5000 µg/mL. A standard 250 µg research dose then requires 0.05 mL (5 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 250 µg draw volume at common BAC water volumes for a 10 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 10000 µg/mL | 0.03 mL | 2.5 IU |
| 2 mL Standard | 5000 µg/mL | 0.05 mL | 5 IU |
| 3 mL | 3333.33 µg/mL | 0.08 mL | 7.5 IU |
| 5 mL | 2000 µg/mL | 0.13 mL | 12.5 IU |
Dose volume at the standard 5000 µg/mL concentration
How draw volume changes if you vary the dose at the standard 10 mg + 2 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 125 µg | 0.03 mL | 2.5 IU |
| 250 µg Std | 0.05 mL | 5 IU |
| 375 µg | 0.08 mL | 7.5 IU |
| 500 µg | 0.1 mL | 10 IU |
| 750 µg | 0.15 mL | 15 IU |
Reconstitution steps
- Wipe the top of both the Melanotan II vial and the bacteriostatic water vial with alcohol swabs.
- Draw 2 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the Melanotan II vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2-8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- Melanotan II 10 mg lyophilized vial
- 2 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 10 mg Melanotan II vial?
For the standard ASCEND research protocol, add 2 mL of bacteriostatic water to a 10 mg Melanotan II vial. This yields 5000 µg/mL.
What concentration does 2 mL BAC water give for 10 mg Melanotan II?
A 10 mg Melanotan II vial reconstituted with 2 mL of bacteriostatic water yields 5000 µg/mL. A 250 µg dose then requires 0.05 mL (5 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for Melanotan II?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is Melanotan II stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2-8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for Melanotan II?
For a 250 µg dose (5 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.
Can I transport reconstituted Melanotan II?
Keep it cold in transit. An insulated bag or small cooler with an ice pack holds the 2-8°C range for several hours, which covers most day trips or drives. For longer travel, pack it the same way you would any refrigerated medication, and get it back into a fridge as soon as you arrive. Extended time at room temperature shortens the ~28-day stability window.
Related
MOTS-C calculator + tracker, free on iOS Download on the App Store Get appFor research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org