RECONSTITUTION GUIDE
How Much BAC Water for Kisspeptin-54?
Quick answer: Add 1 mL of bacteriostatic water to a 1 mg Kisspeptin-54 vial. This yields a concentration of 1000 µg/mL. A standard 438 µg research dose then requires 0.44 mL (43.8 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 438 µg draw volume at common BAC water volumes for a 1 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL Standard | 1000 µg/mL | 0.44 mL | 43.8 IU |
| 2 mL | 500 µg/mL | 0.88 mL | 87.6 IU |
| 3 mL | 333.33 µg/mL | 1.31 mL | 131.4 IU |
| 5 mL | 200 µg/mL | 2.19 mL | 219 IU |
Dose volume at the standard 1000 µg/mL concentration
How draw volume changes if you vary the dose at the standard 1 mg + 1 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 219 µg | 0.22 mL | 21.9 IU |
| 438 µg Std | 0.44 mL | 43.8 IU |
| 657 µg | 0.66 mL | 65.7 IU |
| 876 µg | 0.88 mL | 87.6 IU |
| 1314 µg | 1.31 mL | 131.4 IU |
Reconstitution steps
- Wipe the top of both the Kisspeptin-54 vial and the bacteriostatic water vial with alcohol swabs.
- Draw 1 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the Kisspeptin-54 vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- Kisspeptin-54 1 mg lyophilized vial
- 1 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 1 mg Kisspeptin-54 vial?
For the standard ASCEND research protocol, add 1 mL of bacteriostatic water to a 1 mg Kisspeptin-54 vial. This yields 1000 µg/mL.
What concentration does 1 mL BAC water give for 1 mg Kisspeptin-54?
A 1 mg Kisspeptin-54 vial reconstituted with 1 mL of bacteriostatic water yields 1000 µg/mL. A 438 µg dose then requires 0.44 mL (43.8 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for Kisspeptin-54?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is Kisspeptin-54 stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for Kisspeptin-54?
For a 438 µg dose (43.8 IU draw), a 50-unit (0.5 mL) or 100-unit U-100 insulin syringe is appropriate.
Related
For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org