RECONSTITUTION GUIDE
How Much BAC Water for HMG?
Quick answer: Add 1 mL of bacteriostatic water to a 75 IU HMG vial. This yields a concentration of 75 IU/mL. A standard 75 IU research dose then requires 1 mL (100 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 75 IU draw volume at common BAC water volumes for a 75 IU vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL Standard | 75 IU/mL | 1 mL | 100 IU |
| 2 mL | 37.5 IU/mL | 2 mL | 200 IU |
| 3 mL | 25 IU/mL | 3 mL | 300 IU |
| 5 mL | 15 IU/mL | 5 mL | 500 IU |
Dose volume at the standard 75 IU/mL concentration
How draw volume changes if you vary the dose at the standard 75 IU + 1 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 37.5 IU | 0.5 mL | 50 IU |
| 75 IU Std | 1 mL | 100 IU |
| 112.5 IU | 1.5 mL | 150 IU |
| 150 IU | 2 mL | 200 IU |
| 225 IU | 3 mL | 300 IU |
Reconstitution steps
- Wipe the top of both the HMG vial and the bacteriostatic water vial with alcohol swabs.
- Draw 1 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the HMG vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2-8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- HMG 75 IU lyophilized vial
- 1 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 75 IU HMG vial?
For the standard ASCEND research protocol, add 1 mL of bacteriostatic water to a 75 IU HMG vial. This yields 75 IU/mL.
What concentration does 1 mL BAC water give for 75 IU HMG?
A 75 IU HMG vial reconstituted with 1 mL of bacteriostatic water yields 75 IU/mL. A 75 IU dose then requires 1 mL (100 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for HMG?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is HMG stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2-8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for HMG?
A standard 100-unit (1 mL) U-100 insulin syringe is appropriate for a 75 IU HMG dose.
Can I transport reconstituted HMG?
Keep it cold in transit. An insulated bag or small cooler with an ice pack holds the 2-8°C range for several hours, which covers most day trips or drives. For longer travel, pack it the same way you would any refrigerated medication, and get it back into a fridge as soon as you arrive. Extended time at room temperature shortens the ~28-day stability window.
How do I convert IU to mL for HMG?
IU (International Units) and mL are not directly convertible without knowing the reconstituted concentration. Divide your target dose in IU by the concentration in IU/mL to get the draw volume. For example, at 75 IU/mL (this vial's default mix), 10 IU is 0.133 mL, too small to draw accurately on its own, which is why HMG doses are usually a few hundred IU. Enter your own vial strength and water volume in the calculator above for an exact number.
Related
Verify supplements by lot number, free on iOS Download on the App Store Get appFor research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org