RECONSTITUTION GUIDE
How Much BAC Water for HMG?
Quick answer: Add 1 mL of bacteriostatic water to a 75 mg HMG vial. This yields a concentration of 75 IU/mL. A standard 75 IU research dose then requires 1 mL (100 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 75 IU draw volume at common BAC water volumes for a 75 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL Standard | 75 IU/mL | 1 mL | 100 IU |
| 2 mL | 37.5 IU/mL | 2 mL | 200 IU |
| 3 mL | 25 IU/mL | 3 mL | 300 IU |
| 5 mL | 15 IU/mL | 5 mL | 500 IU |
Dose volume at the standard 75 IU/mL concentration
How draw volume changes if you vary the dose at the standard 75 mg + 1 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 37.5 IU | 0.5 mL | 50 IU |
| 75 IU Std | 1 mL | 100 IU |
| 112.5 IU | 1.5 mL | 150 IU |
| 150 IU | 2 mL | 200 IU |
| 225 IU | 3 mL | 300 IU |
Reconstitution steps
- Wipe the top of both the HMG vial and the bacteriostatic water vial with alcohol swabs.
- Draw 1 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the HMG vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- HMG 75 mg lyophilized vial
- 1 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 75 mg HMG vial?
For the standard ASCEND research protocol, add 1 mL of bacteriostatic water to a 75 mg HMG vial. This yields 75 IU/mL.
What concentration does 1 mL BAC water give for 75 mg HMG?
A 75 mg HMG vial reconstituted with 1 mL of bacteriostatic water yields 75 IU/mL. A 75 IU dose then requires 1 mL (100 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for HMG?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is HMG stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for HMG?
A standard 100-unit (1 mL) U-100 insulin syringe is appropriate for a 75 IU HMG dose.
Related
For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org