RECONSTITUTION GUIDE
How Much BAC Water for Glutathione?
Quick answer: Add 2 mL of bacteriostatic water to a 600 mg Glutathione vial. This yields a concentration of 300 mg/mL. A standard 200 mg research dose then requires 0.67 mL (66.7 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 200 mg draw volume at common BAC water volumes for a 600 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 600 mg/mL | 0.33 mL | 33.3 IU |
| 2 mL Standard | 300 mg/mL | 0.67 mL | 66.7 IU |
| 3 mL | 200 mg/mL | 1 mL | 100 IU |
| 5 mL | 120 mg/mL | 1.67 mL | 166.7 IU |
Dose volume at the standard 300 mg/mL concentration
How draw volume changes if you vary the dose at the standard 600 mg + 2 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 100 mg | 0.33 mL | 33.3 IU |
| 200 mg Std | 0.67 mL | 66.7 IU |
| 300 mg | 1 mL | 100 IU |
| 400 mg | 1.33 mL | 133.3 IU |
| 600 mg | 2 mL | 200 IU |
Reconstitution steps
- Wipe the top of both the Glutathione vial and the bacteriostatic water vial with alcohol swabs.
- Draw 2 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the Glutathione vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- Glutathione 600 mg lyophilized vial
- 2 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 600 mg Glutathione vial?
For the standard ASCEND research protocol, add 2 mL of bacteriostatic water to a 600 mg Glutathione vial. This yields 300 mg/mL.
What concentration does 2 mL BAC water give for 600 mg Glutathione?
A 600 mg Glutathione vial reconstituted with 2 mL of bacteriostatic water yields 300 mg/mL. A 200 mg dose then requires 0.67 mL (66.7 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for Glutathione?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is Glutathione stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for Glutathione?
A standard 100-unit (1 mL) U-100 insulin syringe is appropriate for a 200 mg Glutathione dose.
Related
For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org