RECONSTITUTION GUIDE
How Much BAC Water for Tri-Heal?
Quick answer: Add 3 mL of bacteriostatic water to a 45 mg Tri-Heal vial. This yields a concentration of 15000 µg/mL. A standard 1000 µg research dose then requires 0.07 mL (6.7 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 1000 µg draw volume at common BAC water volumes for a 45 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 45000 µg/mL | 0.02 mL | 2.2 IU |
| 2 mL | 22500 µg/mL | 0.04 mL | 4.4 IU |
| 3 mL Standard | 15000 µg/mL | 0.07 mL | 6.7 IU |
| 5 mL | 9000 µg/mL | 0.11 mL | 11.1 IU |
Dose volume at the standard 15000 µg/mL concentration
How draw volume changes if you vary the dose at the standard 45 mg + 3 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 500 µg | 0.03 mL | 3.3 IU |
| 1000 µg Std | 0.07 mL | 6.7 IU |
| 1500 µg | 0.1 mL | 10 IU |
| 2000 µg | 0.13 mL | 13.3 IU |
| 3000 µg | 0.2 mL | 20 IU |
Reconstitution steps
- Wipe the top of both the Tri-Heal vial and the bacteriostatic water vial with alcohol swabs.
- Draw 3 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the Tri-Heal vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- Tri-Heal 45 mg lyophilized vial
- 3 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 45 mg Tri-Heal vial?
For the standard ASCEND research protocol, add 3 mL of bacteriostatic water to a 45 mg Tri-Heal vial. This yields 15000 µg/mL.
What concentration does 3 mL BAC water give for 45 mg Tri-Heal?
A 45 mg Tri-Heal vial reconstituted with 3 mL of bacteriostatic water yields 15000 µg/mL. A 1000 µg dose then requires 0.07 mL (6.7 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for Tri-Heal?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is Tri-Heal stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for Tri-Heal?
For a 1000 µg dose (6.7 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.
Related
For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org