RECONSTITUTION GUIDE

How Much BAC Water for Tirzepatide?

Quick answer: Add 1 mL of bacteriostatic water to a 15 mg Tirzepatide vial. This yields a concentration of 15 mg/mL. A standard 2.5 mg research dose then requires 0.17 mL (16.7 IU on a 100-unit insulin syringe).

2.5 mg draw on a 100-unit insulin syringe

Concentration by BAC water volume

Concentration and 2.5 mg draw volume at common BAC water volumes for a 15 mg vial.

BAC water	Concentration	Dose volume	Draw
1 mL Standard	15 mg/mL	0.17 mL	16.7 IU
2 mL	7.5 mg/mL	0.33 mL	33.3 IU
3 mL	5 mg/mL	0.5 mL	50 IU
5 mL	3 mg/mL	0.83 mL	83.3 IU

Dose volume at the standard 15 mg/mL concentration

How draw volume changes if you vary the dose at the standard 15 mg + 1 mL reconstitution.

Dose	Volume	IU draw (100u)
1.25 mg	0.08 mL	8.3 IU
2.5 mg Std	0.17 mL	16.7 IU
3.75 mg	0.25 mL	25 IU
5 mg	0.33 mL	33.3 IU
7.5 mg	0.5 mL	50 IU

Reconstitution steps

Wipe the top of both the Tirzepatide vial and the bacteriostatic water vial with alcohol swabs.
Draw 1 mL of bacteriostatic water into a sterile syringe.
Inject the water slowly down the inside glass wall of the Tirzepatide vial. Do not aim directly at the powder.
Swirl gently until fully dissolved. Never shake.
Refrigerate at 2-8°C protected from light. Allow to reach room temperature before each draw.

Supplies needed

Tirzepatide 15 mg lyophilized vial
1 mL bacteriostatic water (0.9% benzyl alcohol)
100-unit (1 mL) U-100 insulin syringe
Alcohol swabs
Sharps container for disposal

Open Tirzepatide Calculator → What is bacteriostatic water?

Frequently asked

How much BAC water do I add to a 15 mg Tirzepatide vial?

For the standard ASCEND research protocol, add 1 mL of bacteriostatic water to a 15 mg Tirzepatide vial. This yields 15 mg/mL.

What concentration does 1 mL BAC water give for 15 mg Tirzepatide?

A 15 mg Tirzepatide vial reconstituted with 1 mL of bacteriostatic water yields 15 mg/mL. A 2.5 mg dose then requires 0.17 mL (16.7 IU on a 100-unit insulin syringe).

Can I use sterile water instead of bacteriostatic water for Tirzepatide?

Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.

How long is Tirzepatide stable once reconstituted with BAC water?

Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2-8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.

What size insulin syringe should I use for Tirzepatide?

For a 2.5 mg dose (16.7 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.

Can I transport reconstituted Tirzepatide?

Keep it cold in transit. An insulated bag or small cooler with an ice pack holds the 2-8°C range for several hours, which covers most day trips or drives. For longer travel, pack it the same way you would any refrigerated medication, and get it back into a fridge as soon as you arrive. Extended time at room temperature shortens the ~28-day stability window.

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For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org

Tirzepatide dose calculator