RECONSTITUTION GUIDE
How Much BAC Water for Thymosin Beta-4?
Quick answer: Add 2 mL of bacteriostatic water to a 5 mg Thymosin Beta-4 vial. This yields a concentration of 2.5 mg/mL. A standard 5 mg research dose then requires 2 mL (200 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 5 mg draw volume at common BAC water volumes for a 5 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 5 mg/mL | 1 mL | 100 IU |
| 2 mL Standard | 2.5 mg/mL | 2 mL | 200 IU |
| 3 mL | 1.67 mg/mL | 3 mL | 300 IU |
| 5 mL | 1 mg/mL | 5 mL | 500 IU |
Dose volume at the standard 2.5 mg/mL concentration
How draw volume changes if you vary the dose at the standard 5 mg + 2 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 2.5 mg | 1 mL | 100 IU |
| 5 mg Std | 2 mL | 200 IU |
| 7.5 mg | 3 mL | 300 IU |
| 10 mg | 4 mL | 400 IU |
| 15 mg | 6 mL | 600 IU |
Reconstitution steps
- Wipe the top of both the Thymosin Beta-4 vial and the bacteriostatic water vial with alcohol swabs.
- Draw 2 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the Thymosin Beta-4 vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- Thymosin Beta-4 5 mg lyophilized vial
- 2 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 5 mg Thymosin Beta-4 vial?
For the standard ASCEND research protocol, add 2 mL of bacteriostatic water to a 5 mg Thymosin Beta-4 vial. This yields 2.5 mg/mL.
What concentration does 2 mL BAC water give for 5 mg Thymosin Beta-4?
A 5 mg Thymosin Beta-4 vial reconstituted with 2 mL of bacteriostatic water yields 2.5 mg/mL. A 5 mg dose then requires 2 mL (200 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for Thymosin Beta-4?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is Thymosin Beta-4 stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for Thymosin Beta-4?
A standard 100-unit (1 mL) U-100 insulin syringe is appropriate for a 5 mg Thymosin Beta-4 dose.
Related
For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org