RECONSTITUTION GUIDE
How Much BAC Water for Testosterone?
Quick answer: Add 10 mL of bacteriostatic water to a 200 mg Testosterone vial. This yields a concentration of 20 mg/mL. A standard 100 mg research dose then requires 5 mL (500 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 100 mg draw volume at common BAC water volumes for a 200 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 200 mg/mL | 0.5 mL | 50 IU |
| 2 mL | 100 mg/mL | 1 mL | 100 IU |
| 3 mL | 66.67 mg/mL | 1.5 mL | 150 IU |
| 5 mL | 40 mg/mL | 2.5 mL | 250 IU |
Dose volume at the standard 20 mg/mL concentration
How draw volume changes if you vary the dose at the standard 200 mg + 10 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 50 mg | 2.5 mL | 250 IU |
| 100 mg Std | 5 mL | 500 IU |
| 150 mg | 7.5 mL | 750 IU |
| 200 mg | 10 mL | 1000 IU |
| 300 mg | 15 mL | 1500 IU |
Reconstitution steps
- Wipe the top of both the Testosterone vial and the bacteriostatic water vial with alcohol swabs.
- Draw 10 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the Testosterone vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2-8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- Testosterone 200 mg lyophilized vial
- 10 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 200 mg Testosterone vial?
For the standard ASCEND research protocol, add 10 mL of bacteriostatic water to a 200 mg Testosterone vial. This yields 20 mg/mL.
What concentration does 10 mL BAC water give for 200 mg Testosterone?
A 200 mg Testosterone vial reconstituted with 10 mL of bacteriostatic water yields 20 mg/mL. A 100 mg dose then requires 5 mL (500 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for Testosterone?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is Testosterone stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2-8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for Testosterone?
A standard 100-unit (1 mL) U-100 insulin syringe is appropriate for a 100 mg Testosterone dose.
Can I transport reconstituted Testosterone?
Keep it cold in transit. An insulated bag or small cooler with an ice pack holds the 2-8°C range for several hours, which covers most day trips or drives. For longer travel, pack it the same way you would any refrigerated medication, and get it back into a fridge as soon as you arrive. Extended time at room temperature shortens the ~28-day stability window.
Related
GHK-Cu calculator + tracker, free on iOS Download on the App Store Get appFor research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org