RECONSTITUTION GUIDE

How Much BAC Water for PE 22-28?

Quick answer: Add 2 mL of bacteriostatic water to a 5 mg PE 22-28 vial. This yields a concentration of 2500 µg/mL. A standard 400 µg research dose then requires 0.16 mL (16 IU on a 100-unit insulin syringe).

400 µg draw on a 100-unit insulin syringe

Concentration by BAC water volume

Concentration and 400 µg draw volume at common BAC water volumes for a 5 mg vial.

BAC water	Concentration	Dose volume	Draw
1 mL	5000 µg/mL	0.08 mL	8 IU
2 mL Standard	2500 µg/mL	0.16 mL	16 IU
3 mL	1666.67 µg/mL	0.24 mL	24 IU
5 mL	1000 µg/mL	0.4 mL	40 IU

Dose volume at the standard 2500 µg/mL concentration

How draw volume changes if you vary the dose at the standard 5 mg + 2 mL reconstitution.

Dose	Volume	IU draw (100u)
200 µg	0.08 mL	8 IU
400 µg Std	0.16 mL	16 IU
600 µg	0.24 mL	24 IU
800 µg	0.32 mL	32 IU
1200 µg	0.48 mL	48 IU

Reconstitution steps

Wipe the top of both the PE 22-28 vial and the bacteriostatic water vial with alcohol swabs.
Draw 2 mL of bacteriostatic water into a sterile syringe.
Inject the water slowly down the inside glass wall of the PE 22-28 vial. Do not aim directly at the powder.
Swirl gently until fully dissolved. Never shake.
Refrigerate at 2-8°C protected from light. Allow to reach room temperature before each draw.

Supplies needed

PE 22-28 5 mg lyophilized vial
2 mL bacteriostatic water (0.9% benzyl alcohol)
100-unit (1 mL) U-100 insulin syringe
Alcohol swabs
Sharps container for disposal

Open PE 22-28 Calculator → What is bacteriostatic water?

Frequently asked

How much BAC water do I add to a 5 mg PE 22-28 vial?

For the standard ASCEND research protocol, add 2 mL of bacteriostatic water to a 5 mg PE 22-28 vial. This yields 2500 µg/mL.

What concentration does 2 mL BAC water give for 5 mg PE 22-28?

A 5 mg PE 22-28 vial reconstituted with 2 mL of bacteriostatic water yields 2500 µg/mL. A 400 µg dose then requires 0.16 mL (16 IU on a 100-unit insulin syringe).

Can I use sterile water instead of bacteriostatic water for PE 22-28?

Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.

How long is PE 22-28 stable once reconstituted with BAC water?

Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2-8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.

What size insulin syringe should I use for PE 22-28?

For a 400 µg dose (16 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.

Can I transport reconstituted PE 22-28?

Keep it cold in transit. An insulated bag or small cooler with an ice pack holds the 2-8°C range for several hours, which covers most day trips or drives. For longer travel, pack it the same way you would any refrigerated medication, and get it back into a fridge as soon as you arrive. Extended time at room temperature shortens the ~28-day stability window.

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For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org

PE 22-28 dose calculator