RECONSTITUTION GUIDE
How Much BAC Water for PE 22-28?
Quick answer: Add 2 mL of bacteriostatic water to a 5 mg PE 22-28 vial. This yields a concentration of 2500 µg/mL. A standard 400 µg research dose then requires 0.16 mL (16 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 400 µg draw volume at common BAC water volumes for a 5 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 5000 µg/mL | 0.08 mL | 8 IU |
| 2 mL Standard | 2500 µg/mL | 0.16 mL | 16 IU |
| 3 mL | 1666.67 µg/mL | 0.24 mL | 24 IU |
| 5 mL | 1000 µg/mL | 0.4 mL | 40 IU |
Dose volume at the standard 2500 µg/mL concentration
How draw volume changes if you vary the dose at the standard 5 mg + 2 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 200 µg | 0.08 mL | 8 IU |
| 400 µg Std | 0.16 mL | 16 IU |
| 600 µg | 0.24 mL | 24 IU |
| 800 µg | 0.32 mL | 32 IU |
| 1200 µg | 0.48 mL | 48 IU |
Reconstitution steps
- Wipe the top of both the PE 22-28 vial and the bacteriostatic water vial with alcohol swabs.
- Draw 2 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the PE 22-28 vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- PE 22-28 5 mg lyophilized vial
- 2 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 5 mg PE 22-28 vial?
For the standard ASCEND research protocol, add 2 mL of bacteriostatic water to a 5 mg PE 22-28 vial. This yields 2500 µg/mL.
What concentration does 2 mL BAC water give for 5 mg PE 22-28?
A 5 mg PE 22-28 vial reconstituted with 2 mL of bacteriostatic water yields 2500 µg/mL. A 400 µg dose then requires 0.16 mL (16 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for PE 22-28?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is PE 22-28 stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for PE 22-28?
For a 400 µg dose (16 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.
Related
For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org