RECONSTITUTION GUIDE

How Much BAC Water for Neuroxelin?

Quick answer: Add 3 mL of bacteriostatic water to a 48 mg Neuroxelin vial. This yields a concentration of 16000 µg/mL. A standard 500 µg research dose then requires 0.03 mL (3.1 IU on a 100-unit insulin syringe).

500 µg draw on a 100-unit insulin syringe
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Concentration by BAC water volume

Concentration and 500 µg draw volume at common BAC water volumes for a 48 mg vial.

BAC waterConcentrationDose volumeDraw
1 mL 48000 µg/mL 0.01 mL 1 IU
2 mL 24000 µg/mL 0.02 mL 2.1 IU
3 mL Standard 16000 µg/mL 0.03 mL 3.1 IU
5 mL 9600 µg/mL 0.05 mL 5.2 IU

Dose volume at the standard 16000 µg/mL concentration

How draw volume changes if you vary the dose at the standard 48 mg + 3 mL reconstitution.

DoseVolumeIU draw (100u)
250 µg 0.02 mL 1.6 IU
500 µg Std 0.03 mL 3.1 IU
750 µg 0.05 mL 4.7 IU
1000 µg 0.06 mL 6.3 IU
1500 µg 0.09 mL 9.4 IU

Reconstitution steps

  1. Wipe the top of both the Neuroxelin vial and the bacteriostatic water vial with alcohol swabs.
  2. Draw 3 mL of bacteriostatic water into a sterile syringe.
  3. Inject the water slowly down the inside glass wall of the Neuroxelin vial. Do not aim directly at the powder.
  4. Swirl gently until fully dissolved. Never shake.
  5. Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.

Supplies needed

Open Neuroxelin Calculator → What is bacteriostatic water?

Frequently asked

How much BAC water do I add to a 48 mg Neuroxelin vial?
For the standard ASCEND research protocol, add 3 mL of bacteriostatic water to a 48 mg Neuroxelin vial. This yields 16000 µg/mL.
What concentration does 3 mL BAC water give for 48 mg Neuroxelin?
A 48 mg Neuroxelin vial reconstituted with 3 mL of bacteriostatic water yields 16000 µg/mL. A 500 µg dose then requires 0.03 mL (3.1 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for Neuroxelin?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is Neuroxelin stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for Neuroxelin?
For a 500 µg dose (3.1 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.

Related

For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org