RECONSTITUTION GUIDE

How Much BAC Water for Methylcobalamin?

Quick answer: Add 10 mL of bacteriostatic water to a 10 mg Methylcobalamin vial. This yields a concentration of 1 mg/mL. A standard 1 mg research dose then requires 1 mL (100 IU on a 100-unit insulin syringe).

1 mg draw on a 100-unit insulin syringe

Concentration by BAC water volume

Concentration and 1 mg draw volume at common BAC water volumes for a 10 mg vial.

BAC water	Concentration	Dose volume	Draw
1 mL	10 mg/mL	0.1 mL	10 IU
2 mL	5 mg/mL	0.2 mL	20 IU
3 mL	3.33 mg/mL	0.3 mL	30 IU
5 mL	2 mg/mL	0.5 mL	50 IU

Dose volume at the standard 1 mg/mL concentration

How draw volume changes if you vary the dose at the standard 10 mg + 10 mL reconstitution.

Dose	Volume	IU draw (100u)
0.5 mg	0.5 mL	50 IU
1 mg Std	1 mL	100 IU
1.5 mg	1.5 mL	150 IU
2 mg	2 mL	200 IU
3 mg	3 mL	300 IU

Reconstitution steps

Wipe the top of both the Methylcobalamin vial and the bacteriostatic water vial with alcohol swabs.
Draw 10 mL of bacteriostatic water into a sterile syringe.
Inject the water slowly down the inside glass wall of the Methylcobalamin vial. Do not aim directly at the powder.
Swirl gently until fully dissolved. Never shake.
Refrigerate at 2-8°C protected from light. Allow to reach room temperature before each draw.

Supplies needed

Methylcobalamin 10 mg lyophilized vial
10 mL bacteriostatic water (0.9% benzyl alcohol)
100-unit (1 mL) U-100 insulin syringe
Alcohol swabs
Sharps container for disposal

Open Methylcobalamin Calculator → What is bacteriostatic water?

Frequently asked

How much BAC water do I add to a 10 mg Methylcobalamin vial?

For the standard ASCEND research protocol, add 10 mL of bacteriostatic water to a 10 mg Methylcobalamin vial. This yields 1 mg/mL.

What concentration does 10 mL BAC water give for 10 mg Methylcobalamin?

A 10 mg Methylcobalamin vial reconstituted with 10 mL of bacteriostatic water yields 1 mg/mL. A 1 mg dose then requires 1 mL (100 IU on a 100-unit insulin syringe).

Can I use sterile water instead of bacteriostatic water for Methylcobalamin?

Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.

How long is Methylcobalamin stable once reconstituted with BAC water?

Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2-8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.

What size insulin syringe should I use for Methylcobalamin?

A standard 100-unit (1 mL) U-100 insulin syringe is appropriate for a 1 mg Methylcobalamin dose.

Can I transport reconstituted Methylcobalamin?

Keep it cold in transit. An insulated bag or small cooler with an ice pack holds the 2-8°C range for several hours, which covers most day trips or drives. For longer travel, pack it the same way you would any refrigerated medication, and get it back into a fridge as soon as you arrive. Extended time at room temperature shortens the ~28-day stability window.

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For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org

Methylcobalamin dose calculator