RECONSTITUTION GUIDE
How Much BAC Water for Livagen?
Quick answer: Add 3 mL of bacteriostatic water to a 20 mg Livagen vial. This yields a concentration of 6666.67 µg/mL. A standard 500 µg research dose then requires 0.08 mL (7.5 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 500 µg draw volume at common BAC water volumes for a 20 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 20000 µg/mL | 0.03 mL | 2.5 IU |
| 2 mL | 10000 µg/mL | 0.05 mL | 5 IU |
| 3 mL Standard | 6666.67 µg/mL | 0.08 mL | 7.5 IU |
| 5 mL | 4000 µg/mL | 0.13 mL | 12.5 IU |
Dose volume at the standard 6666.67 µg/mL concentration
How draw volume changes if you vary the dose at the standard 20 mg + 3 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 250 µg | 0.04 mL | 3.8 IU |
| 500 µg Std | 0.08 mL | 7.5 IU |
| 750 µg | 0.11 mL | 11.2 IU |
| 1000 µg | 0.15 mL | 15 IU |
| 1500 µg | 0.23 mL | 22.5 IU |
Reconstitution steps
- Wipe the top of both the Livagen vial and the bacteriostatic water vial with alcohol swabs.
- Draw 3 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the Livagen vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- Livagen 20 mg lyophilized vial
- 3 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 20 mg Livagen vial?
For the standard ASCEND research protocol, add 3 mL of bacteriostatic water to a 20 mg Livagen vial. This yields 6666.67 µg/mL.
What concentration does 3 mL BAC water give for 20 mg Livagen?
A 20 mg Livagen vial reconstituted with 3 mL of bacteriostatic water yields 6666.67 µg/mL. A 500 µg dose then requires 0.08 mL (7.5 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for Livagen?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is Livagen stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for Livagen?
For a 500 µg dose (7.5 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.
Related
For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org