RECONSTITUTION GUIDE

How Much BAC Water for Klotho?

Quick answer: Add 1 mL of bacteriostatic water to a 1 mg Klotho vial. This yields a concentration of 1000 µg/mL. A standard 2 µg research dose then requires 0 mL (0.2 IU on a 100-unit insulin syringe).

2 µg draw on a 100-unit insulin syringe

Concentration by BAC water volume

Concentration and 2 µg draw volume at common BAC water volumes for a 1 mg vial.

BAC water	Concentration	Dose volume	Draw
1 mL Standard	1000 µg/mL	0 mL	0.2 IU
2 mL	500 µg/mL	0 mL	0.4 IU
3 mL	333.33 µg/mL	0.01 mL	0.6 IU
5 mL	200 µg/mL	0.01 mL	1 IU

Dose volume at the standard 1000 µg/mL concentration

How draw volume changes if you vary the dose at the standard 1 mg + 1 mL reconstitution.

Dose	Volume	IU draw (100u)
1 µg	0 mL	0.1 IU
2 µg Std	0 mL	0.2 IU
3 µg	0 mL	0.3 IU
4 µg	0 mL	0.4 IU
6 µg	0.01 mL	0.6 IU

Reconstitution steps

Wipe the top of both the Klotho vial and the bacteriostatic water vial with alcohol swabs.
Draw 1 mL of bacteriostatic water into a sterile syringe.
Inject the water slowly down the inside glass wall of the Klotho vial. Do not aim directly at the powder.
Swirl gently until fully dissolved. Never shake.
Refrigerate at 2-8°C protected from light. Allow to reach room temperature before each draw.

Supplies needed

Klotho 1 mg lyophilized vial
1 mL bacteriostatic water (0.9% benzyl alcohol)
100-unit (1 mL) U-100 insulin syringe
Alcohol swabs
Sharps container for disposal

Open Klotho Calculator → What is bacteriostatic water?

Frequently asked

How much BAC water do I add to a 1 mg Klotho vial?

For the standard ASCEND research protocol, add 1 mL of bacteriostatic water to a 1 mg Klotho vial. This yields 1000 µg/mL.

What concentration does 1 mL BAC water give for 1 mg Klotho?

A 1 mg Klotho vial reconstituted with 1 mL of bacteriostatic water yields 1000 µg/mL. A 2 µg dose then requires 0 mL (0.2 IU on a 100-unit insulin syringe).

Can I use sterile water instead of bacteriostatic water for Klotho?

Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.

How long is Klotho stable once reconstituted with BAC water?

Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2-8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.

What size insulin syringe should I use for Klotho?

For a 2 µg dose (0.2 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.

Can I transport reconstituted Klotho?

Keep it cold in transit. An insulated bag or small cooler with an ice pack holds the 2-8°C range for several hours, which covers most day trips or drives. For longer travel, pack it the same way you would any refrigerated medication, and get it back into a fridge as soon as you arrive. Extended time at room temperature shortens the ~28-day stability window.

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For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org

Klotho dose calculator