RECONSTITUTION GUIDE

How Much BAC Water for Humanin?

Quick answer: Add 2 mL of bacteriostatic water to a 5 mg Humanin vial. This yields a concentration of 2.5 mg/mL. A standard 2 mg research dose then requires 0.8 mL (80 IU on a 100-unit insulin syringe).

2 mg draw on a 100-unit insulin syringe

Concentration by BAC water volume

Concentration and 2 mg draw volume at common BAC water volumes for a 5 mg vial.

BAC water	Concentration	Dose volume	Draw
1 mL	5 mg/mL	0.4 mL	40 IU
2 mL Standard	2.5 mg/mL	0.8 mL	80 IU
3 mL	1.67 mg/mL	1.2 mL	120 IU
5 mL	1 mg/mL	2 mL	200 IU

Dose volume at the standard 2.5 mg/mL concentration

How draw volume changes if you vary the dose at the standard 5 mg + 2 mL reconstitution.

Dose	Volume	IU draw (100u)
1 mg	0.4 mL	40 IU
2 mg Std	0.8 mL	80 IU
3 mg	1.2 mL	120 IU
4 mg	1.6 mL	160 IU
6 mg	2.4 mL	240 IU

Reconstitution steps

Wipe the top of both the Humanin vial and the bacteriostatic water vial with alcohol swabs.
Draw 2 mL of bacteriostatic water into a sterile syringe.
Inject the water slowly down the inside glass wall of the Humanin vial. Do not aim directly at the powder.
Swirl gently until fully dissolved. Never shake.
Refrigerate at 2-8°C protected from light. Allow to reach room temperature before each draw.

Supplies needed

Humanin 5 mg lyophilized vial
2 mL bacteriostatic water (0.9% benzyl alcohol)
100-unit (1 mL) U-100 insulin syringe
Alcohol swabs
Sharps container for disposal

Open Humanin Calculator → What is bacteriostatic water?

Frequently asked

How much BAC water do I add to a 5 mg Humanin vial?

For the standard ASCEND research protocol, add 2 mL of bacteriostatic water to a 5 mg Humanin vial. This yields 2.5 mg/mL.

What concentration does 2 mL BAC water give for 5 mg Humanin?

A 5 mg Humanin vial reconstituted with 2 mL of bacteriostatic water yields 2.5 mg/mL. A 2 mg dose then requires 0.8 mL (80 IU on a 100-unit insulin syringe).

Can I use sterile water instead of bacteriostatic water for Humanin?

Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.

How long is Humanin stable once reconstituted with BAC water?

Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2-8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.

What size insulin syringe should I use for Humanin?

A standard 100-unit (1 mL) U-100 insulin syringe is appropriate for a 2 mg Humanin dose.

Can I transport reconstituted Humanin?

Keep it cold in transit. An insulated bag or small cooler with an ice pack holds the 2-8°C range for several hours, which covers most day trips or drives. For longer travel, pack it the same way you would any refrigerated medication, and get it back into a fridge as soon as you arrive. Extended time at room temperature shortens the ~28-day stability window.

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For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org

Humanin dose calculator