RECONSTITUTION GUIDE
How Much BAC Water for HCG?
Quick answer: Add 2 mL of bacteriostatic water to a 5000 mg HCG vial. This yields a concentration of 2500 IU/mL. A standard 250 IU research dose then requires 0.1 mL (10 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 250 IU draw volume at common BAC water volumes for a 5000 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 5000 IU/mL | 0.05 mL | 5 IU |
| 2 mL Standard | 2500 IU/mL | 0.1 mL | 10 IU |
| 3 mL | 1666.67 IU/mL | 0.15 mL | 15 IU |
| 5 mL | 1000 IU/mL | 0.25 mL | 25 IU |
Dose volume at the standard 2500 IU/mL concentration
How draw volume changes if you vary the dose at the standard 5000 mg + 2 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 125 IU | 0.05 mL | 5 IU |
| 250 IU Std | 0.1 mL | 10 IU |
| 375 IU | 0.15 mL | 15 IU |
| 500 IU | 0.2 mL | 20 IU |
| 750 IU | 0.3 mL | 30 IU |
Reconstitution steps
- Wipe the top of both the HCG vial and the bacteriostatic water vial with alcohol swabs.
- Draw 2 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the HCG vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- HCG 5000 mg lyophilized vial
- 2 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 5000 mg HCG vial?
For the standard ASCEND research protocol, add 2 mL of bacteriostatic water to a 5000 mg HCG vial. This yields 2500 IU/mL.
What concentration does 2 mL BAC water give for 5000 mg HCG?
A 5000 mg HCG vial reconstituted with 2 mL of bacteriostatic water yields 2500 IU/mL. A 250 IU dose then requires 0.1 mL (10 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for HCG?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is HCG stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for HCG?
For a 250 IU dose (10 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.
Related
For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org