RECONSTITUTION GUIDE
How Much BAC Water for GHK-Cu?
Quick answer: Add 2 mL of bacteriostatic water to a 50 mg GHK-Cu vial. This yields a concentration of 25 mg/mL. A standard 2 mg research dose then requires 0.08 mL (8 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 2 mg draw volume at common BAC water volumes for a 50 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 50 mg/mL | 0.04 mL | 4 IU |
| 2 mL Standard | 25 mg/mL | 0.08 mL | 8 IU |
| 3 mL | 16.67 mg/mL | 0.12 mL | 12 IU |
| 5 mL | 10 mg/mL | 0.2 mL | 20 IU |
Dose volume at the standard 25 mg/mL concentration
How draw volume changes if you vary the dose at the standard 50 mg + 2 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 1 mg | 0.04 mL | 4 IU |
| 2 mg Std | 0.08 mL | 8 IU |
| 3 mg | 0.12 mL | 12 IU |
| 4 mg | 0.16 mL | 16 IU |
| 6 mg | 0.24 mL | 24 IU |
Reconstitution steps
- Wipe the top of both the GHK-Cu vial and the bacteriostatic water vial with alcohol swabs.
- Draw 2 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the GHK-Cu vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2-8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- GHK-Cu 50 mg lyophilized vial
- 2 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 50 mg GHK-Cu vial?
For the standard ASCEND research protocol, add 2 mL of bacteriostatic water to a 50 mg GHK-Cu vial. This yields 25 mg/mL.
What concentration does 2 mL BAC water give for 50 mg GHK-Cu?
A 50 mg GHK-Cu vial reconstituted with 2 mL of bacteriostatic water yields 25 mg/mL. A 2 mg dose then requires 0.08 mL (8 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for GHK-Cu?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is GHK-Cu stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2-8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for GHK-Cu?
For a 2 mg dose (8 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.
Can I transport reconstituted GHK-Cu?
Keep it cold in transit. An insulated bag or small cooler with an ice pack holds the 2-8°C range for several hours, which covers most day trips or drives. For longer travel, pack it the same way you would any refrigerated medication, and get it back into a fridge as soon as you arrive. Extended time at room temperature shortens the ~28-day stability window.
Related
GHK-Cu calculator + tracker, free on iOS Download on the App Store Get appFor research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org