RECONSTITUTION GUIDE
How Much BAC Water for BPC-157?
Quick answer: Add 2 mL of bacteriostatic water to a 5 mg BPC-157 vial. This yields a concentration of 2500 µg/mL. A standard 500 µg research dose then requires 0.2 mL (20 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 500 µg draw volume at common BAC water volumes for a 5 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL | 5000 µg/mL | 0.1 mL | 10 IU |
| 2 mL Standard | 2500 µg/mL | 0.2 mL | 20 IU |
| 3 mL | 1666.67 µg/mL | 0.3 mL | 30 IU |
| 5 mL | 1000 µg/mL | 0.5 mL | 50 IU |
Dose volume at the standard 2500 µg/mL concentration
How draw volume changes if you vary the dose at the standard 5 mg + 2 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 250 µg | 0.1 mL | 10 IU |
| 500 µg Std | 0.2 mL | 20 IU |
| 750 µg | 0.3 mL | 30 IU |
| 1000 µg | 0.4 mL | 40 IU |
| 1500 µg | 0.6 mL | 60 IU |
Reconstitution steps
- Wipe the top of both the BPC-157 vial and the bacteriostatic water vial with alcohol swabs.
- Draw 2 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the BPC-157 vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- BPC-157 5 mg lyophilized vial
- 2 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 5 mg BPC-157 vial?
For the standard ASCEND research protocol, add 2 mL of bacteriostatic water to a 5 mg BPC-157 vial. This yields 2500 µg/mL.
What concentration does 2 mL BAC water give for 5 mg BPC-157?
A 5 mg BPC-157 vial reconstituted with 2 mL of bacteriostatic water yields 2500 µg/mL. A 500 µg dose then requires 0.2 mL (20 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for BPC-157?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is BPC-157 stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for BPC-157?
For a 500 µg dose (20 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.
Related
For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org