RECONSTITUTION GUIDE
How Much BAC Water for ARA-290?
Quick answer: Add 1 mL of bacteriostatic water to a 16 mg ARA-290 vial. This yields a concentration of 16 mg/mL. A standard 4 mg research dose then requires 0.25 mL (25 IU on a 100-unit insulin syringe).
Concentration by BAC water volume
Concentration and 4 mg draw volume at common BAC water volumes for a 16 mg vial.
| BAC water | Concentration | Dose volume | Draw |
|---|---|---|---|
| 1 mL Standard | 16 mg/mL | 0.25 mL | 25 IU |
| 2 mL | 8 mg/mL | 0.5 mL | 50 IU |
| 3 mL | 5.33 mg/mL | 0.75 mL | 75 IU |
| 5 mL | 3.2 mg/mL | 1.25 mL | 125 IU |
Dose volume at the standard 16 mg/mL concentration
How draw volume changes if you vary the dose at the standard 16 mg + 1 mL reconstitution.
| Dose | Volume | IU draw (100u) |
|---|---|---|
| 2 mg | 0.13 mL | 12.5 IU |
| 4 mg Std | 0.25 mL | 25 IU |
| 6 mg | 0.38 mL | 37.5 IU |
| 8 mg | 0.5 mL | 50 IU |
| 12 mg | 0.75 mL | 75 IU |
Reconstitution steps
- Wipe the top of both the ARA-290 vial and the bacteriostatic water vial with alcohol swabs.
- Draw 1 mL of bacteriostatic water into a sterile syringe.
- Inject the water slowly down the inside glass wall of the ARA-290 vial. Do not aim directly at the powder.
- Swirl gently until fully dissolved. Never shake.
- Refrigerate at 2–8°C protected from light. Allow to reach room temperature before each draw.
Supplies needed
- ARA-290 16 mg lyophilized vial
- 1 mL bacteriostatic water (0.9% benzyl alcohol)
- 100-unit (1 mL) U-100 insulin syringe
- Alcohol swabs
- Sharps container for disposal
Frequently asked
How much BAC water do I add to a 16 mg ARA-290 vial?
For the standard ASCEND research protocol, add 1 mL of bacteriostatic water to a 16 mg ARA-290 vial. This yields 16 mg/mL.
What concentration does 1 mL BAC water give for 16 mg ARA-290?
A 16 mg ARA-290 vial reconstituted with 1 mL of bacteriostatic water yields 16 mg/mL. A 4 mg dose then requires 0.25 mL (25 IU on a 100-unit insulin syringe).
Can I use sterile water instead of bacteriostatic water for ARA-290?
Bacteriostatic water (0.9% benzyl alcohol) is preferred because it inhibits bacterial growth and allows multi-dose use of the reconstituted vial. Sterile water lacks preservative properties and accelerates peptide degradation once the vial is opened.
How long is ARA-290 stable once reconstituted with BAC water?
Most research peptides remain stable for approximately 28 days when reconstituted with bacteriostatic water and stored at 2–8°C protected from light. Refer to peptide-specific stability data for any deviation from this baseline.
What size insulin syringe should I use for ARA-290?
For a 4 mg dose (25 IU draw), a 30-unit (0.3 mL) U-100 insulin syringe gives the finest resolution. A 100-unit syringe also works.
Related
For research use only. Not medical advice. Consult a qualified healthcare provider before using any peptide compound. Reconstitution parameters cross-referenced with independent 3rd-party analytical laboratories. · ASCEND · ascendpeptide.org